Using a new technique, researchers have found tiny and previously undetectable “hot spots” of extremely high stiffness inside aggressive and invasive breast cancer tumors.
Their findings suggest, for the first time, that only very tiny regions of a tumor need to stiffen for metastasis to take place. Though still in its infancy, the researchers believe that their technique may prove useful in detecting and mapping the progression of aggressive cancers.
The unusual technique allows researchers to sense, from the perspective of a cancer cell, what is going on in their surrounding environment.
“We are now able to see these features because our approach allows us to take measurements within living, intact, 3D tissues,” says Chris Moraes of the chemical engineering department at McGill University and senior author of the paper in Nature Communications.
“When tissue samples are disrupted in any way, as is normally required with standard techniques, signs of these ‘hot spots’ are eliminated.”
The researchers built tiny hydrogel sensors that can expand on demand, much like inflating balloons the size of individual cells, and placed them inside 3D cultures and mouse models of breast cancer. When triggered, the expansion of the hydrogel can be used to measure very local stiffness inside the tumor.
The unusual technique allows the researchers to sense, from the perspective of a cancer cell, what is going on in their surrounding environment.
“Human cells are not static. They grab and pull on the tissue around them, checking out how rigid or soft their surroundings are. What cells feel around them typically drives their behavior: immune cells can activate, stem cells can become specialized, and cancer cells can become dangerously aggressive,” Moraes says.
“Breast cancer cells usually feel surroundings that are quite soft. However, we found that cancer cells inside aggressive tumors experienced much harder surroundings than previously expected, as hard as really old and dried up gummy bears.”
The researchers believe their findings suggest new ways in which cell mechanics, even at the early stages of breast cancer, might affect disease progression.
“Developing methods to analyze the mechanical profiles in 3D tissues may better predict patient risk and outcome,” says first author Stephanie Mok, a PhD candidate in the chemical engineering department.
“Whether these ‘hot spots’ of stiffness are really causing cancer progression rather than simply being correlated with it remains an open, but critically important question to resolve.”
Source: McGill University